PRR Compound Screening and Profiling Service
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
|
Compound Profiling Single dose testing on a set of PRRs |
Show product |
1 test |
tlrl-test1
|
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|
Compound Dose Response Dose response on one or several PRRs |
Show product |
1 test |
tlrl-test2
|
Custom Screening and Profiling Services for immune-modulating compounds
With over 25 years of experience, InvivoGen provides specialized PRR compound screening services using highly sensitive cell-based assays. Our engineered cell lines are designed to provide conclusive data for identifying lead compounds with immune-modulating properties.
The PRR custom screening and profiling service supports your research in areas such as infectious diseases, cancer, and autoimmune disorders. With decades of expertise, we provide high-quality, reproducible results to support your immune-based drug discovery and vaccine development journey.
Key Features
Fully customizable - To fit your specific research need
Dedicated support team - To answer your questions
Fast results - Delivered within 4 weeks
Affordable - Minimal price for maximal services
Description
PRR reporter signaling pathway
InvivoGen's screening assays enable the reliable detection of activators or inhibitors across key innate immune pathways, including Toll-like receptors (TLRs), NOD-like receptors (NLRs), C-type lectin receptors (CLRs), Stimulator of interferon genes (STING), Retinoic acid-inducible gene I (RIG-I), and Melanoma differentiation-associated protein 5 (MDA5). These sensitive assays are based on engineered HEK293, THP-1, or A549 cells expressing one or two inducible reporter systems: an NF-κB-inducible SEAP (secreted embryonic alkaline phosphatase) and/or an IRF (interferon regulatory factor)-inducible secreted Lucia® luciferase. InvivoGen's proprietary methods detect NF-κB and/or IRF pathway activation or inhibition by your compounds of choice.
Our fully customizable services provide tailored solutions to meet your specific research needs, with a detailed report delivered within just weeks. Fill out the request form and contact us to get in touch.
The prices shown reflect our most commonly requested panels and services. Customizations and alternative options may result in different pricing — see FAQ for details.
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Service options
InvivoGen offers two flexible screening levels, which can be ordered sequentially or individually, depending on your research needs.
- Level 1 - Compound Profiling:
Single-dose testing across one or more selected panels of PRRs to evaluate initial activity. Testing is performed in triplicate, typically at a 1/10 dilution or a concentration specified by the client.
- Level 2 - Compound Dose Response:
Multi-dose testing on specifically chosen PRRs to assess dose dependency. Common concentrations include 1/10, 1/100, and 1/1000 dilutions, with further customization available upon request.
✔ Levels 1 and 2 are suitable for agonist and antagonist screenings.
✔ A detailed report is prepared with complete data analysis.
✔ All procedures are performed according to strict guidelines.
✔ Confidentiality is guaranteed.
Back to the topService step by step
1. Reach out to tech@invivogen.com to discuss your project needs. Complete our Screening Request Form and our team will guide you in selecting the most relevant PRR panel for your therapeutic candidates.
2. We will provide a price quote for your selected screening services. Our lab will review the request and follow up with any questions or clarifications.
3. Once your panel selection and quote are confirmed, send your samples to us. We’ll handle the preparation and set up your compounds for the screening.
4. Our PRR screening services use engineered cell lines in a controlled 96-well format, testing each compound in triplicate to ensure consistency and reliability.
5. After running the assays, our specialists prepare a finalized written report.
6. Our team is available to discuss the results and offer insights on potential next steps in your drug discovery process.
Illustrations on this website are created with BioRender.com.
Back to the topPRR screening panels
Our screening service offers a variety of PRR assays, ensuring tailored solutions for each research goal. Choose from human and mouse PRRs, with options that span critical immune pathways:
| Standard Panel Cell Lines | Positive Control Ligands | Pathway / Reporter | |
|---|---|---|---|
|
TLR Ligand Screening - Human or mouse - |
HEK-Blue™ TLR2 |
Pam3CSK4 |
NF-κB / SEAP
(NF-κB / SEAP and IRF / Lucia |
|
Specific TLR2 Ligand Screening - Human - |
HEK-Blue™ TLR2 HEK-Blue™ TLR2-TLR1 HEK-Blue™ TLR2-TLR6 HEK-Blue™ TLR2 KO-TLR2/6 HEK-Blue™ Null control |
CUT-12-9 CUT-12-9 FSL-1 Human TNF-α Human TNF-α |
NF-κB / SEAP |
|
NOD Ligand Screening - Human or mouse - |
HEK-Blue™ NOD1 HEK-Blue™ NOD2 HEK-Blue™ Null control |
C12-iE-DAP L18-MDP Human TNF-α |
NF-κB / SEAP |
|
CLR Ligand Screening - Human or mouse - |
HEK-Blue™ Dectin1a HEK-Blue™ Dectin1b HEK-Blue™ Dectin2 (optional) HEK-Blue™ Mincle HEK-Blue™ Null control |
WGPS / Zymosan Depleted WGPS / Zymosan Depleted HKCA TDB Human TNF-α |
NF-κB / SEAP |
|
STING Ligand Screening - Human or mouse - |
THP1-Dual™ KO-STING THP1-Dual™ KI-STING variants THP1-Dual™ control |
Human IFN-α / 2'3'-cGAMP Dependent on the STING variant Human IFN-α / 2'3'-cGAMP |
NF-κB / SEAP and IRF / Lucia |
|
RIG-I Ligand Screening - Human - |
HEK-Lucia™ RIG-I
THP1-Dual™ KO-RIG-I
A549-Dual™ KO-RIG-I |
Human IFN-α / 3p-hpRNA (complexed)
Human IFN-α / 3p-hpRNA (complexed)
Human IFN-α / 3p-hpRNA (complexed) |
IRF / Lucia NF-κB / SEAP and IRF / Lucia
|
Don't see your target of interest? Contact us. We have customizable options for you.
Back to the topFAQ
Q: How can PRR screening services support drug discovery and vaccine development?
PRR profiling is critical in identifying compounds that influence immune responses, aiding in adjuvant and drug discovery for conditions like cancer, autoimmunity, and infectious diseases. This process accelerates early-stage drug development by providing reliable data on immune pathway modulation.
Q: What is the benefit of a cellular-based compound screening?
The cell-based data provides a more holistic understanding of the biological activity and the resulting cellular responses. This allows more informed decisions to be made about which compounds should enter therapeutic development.
Q: Are custom PRR screening services available for unique research needs?
Absolutely. We offer custom screening options for specialized research requirements. Whether you need unique PRR pathways or specific concentrations, our team can tailor the assay to meet your study’s needs.
Q: I have a compound or extract that I believe has an immunomodulatory effect. Could InvivoGen's screening service give me an answer to the possible activity on potential PRRs?
Yes, of course! Our PRR Screening Service is designed to assess the potential activity of your compounds or extracts on various PRRs, providing valuable insights into their immunomodulatory effects.
Q: Can I test my compounds on receptors not listed here?
Absolutely. InvivoGen offers many reporter cell lines suitable for screening lead compounds. From cytokine signaling to immune checkpoints, our team can tailor the assay to meet your study’s needs.
Q: How much does the screening service cost?
The price depends on many factors, such as the screening level, number of compounds and cell lines, concentration ranges, and grade of customization. The indicated prices (see above) cover:
- Compound Profiling (Level 1): 1 compound at 1 concentration on our TLR panel (7 cell lines) + control cell line.
- Compound Dose Response (Level 2): 1 compound at 5 concentrations on 1 PRR cell line + control cell line.
For more tailored screening options, contact us, share your requirements, and we’ll provide a personalized quote.
Q: How many concentrations should be tested to determine EC50 or IC50?
In our experience, a minimum of 7 concentrations be tested with a series of 1:3 dilutions to sufficiently calculate an EC50 or IC50.
Q: Why choose InvivoGen for compound screening and profiling services?
With over 25 years of expertise, InvivoGen offers robust, sensitive assays and customizable options. Our reliable data analysis, confidentiality, and commitment to quality make us a trusted choice in immunology research services.
Back to the top