Custom Cell Line Development Service

Accelerate Your Research with Customizable Cell Lines

Customizable cell line service overview

InvivoGen's custom cell line development service is designed to support your specific experimental goals, providing stably engineered cell lines tailored to your research applications.
 

Stable cell lines are invaluable tools for drug screening, functional assays, and signaling pathway studies. However, developing them can be time-consuming and complicated. With more than 25 years of experience in upstream biopharmaceutical development, InvivoGen provides outstanding custom cell line services.

We offer a range of cell line modifications and customization options to match your research requirements and save you time. Explore our available formats below for host cell lines, gene expression, reporter systems, promoters, and selection markers. 

Key Features of InvivoGen’s Custom Cell Lines?

Fully customizable - Tailor-made and adaptive to your needs

 High quality - Guaranteed free of viral particles & other contaminants

Robust - Stability tested up to 10 passages

Reliable - Rigorous quality control

Service Workflow

Our custom-developed cell lines are ideal for pathway analysis, activator/inhibitor studies, high-throughput screening systems, reporter assays, antibody-drug screenings (blocking and binding), and therapeutic development. Whether you need a reporter system or gene overexpression, we will work closely with you to ensure the final product is reliable, functional, and ready for your assays. 

Our on-demand cell line service capabilities are broad and also include cytokine reporter cell lines for exotic species beyond human or mouse as well as co-stimulation models combining two cytokine receptors or a cytokine receptor with an immune checkpoint.

With InvivoGen, imagination knows few limits. Just contact us to connect. 

Figures

Overexpression confirmation.  The expression level of the target protein will be analyzed by flow cytometry. Data shows a comparison of three different clones to negative a control cell line.

Dose-response of three different clones to the stimulant of choice. Cells were stimulated with increasing concentrations of the stimulant of choice. After overnight incubation, the induced reporter protein activity (here SEAP) was determined using the corresponding detection reagent (here QUANTI-Blue™ Solution, a SEAP detection reagent). Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Stability of dose-response of three different clones to the stimulant of choice. Cells were stimulated with increasing concentrations of the stimulant of choice at passage 1 (p+1), p+5, p+10, p+15, p+20. After overnight incubation, the induced reporter protein activity (here SEAP) was determined using the corresponding detection reagent (here QUANTI-Blue™ Solution, a SEAP detection reagent). Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Available formats

We offer a variety of options to ensure that your cell line meets the exact specifications required for your research:

• Host Cell Lines – Mammalian cell lines, including HEK293,  A549, THP-1, Jurkat, Raji, RAW 264.7, or B16 cells
   
• Cell Line Modifications – Stable overexpression of gene(s) of interest via transfection or lentiviral transduction
   
• Reporter – One or two inducible or constitutive markers for visualization and quantification, such as SEAP, Lucia® Luciferase, GFP, ACP5, or others
   
• Promoters – Constitutive active or inducible: NF-κB, ISRE, NFAT, STAT1, STAT3, STAT4, STAT5, STAT6, ARE, CREB, and more
   
• Antibiotic Resistance – Zeocin®, Blasticidin, Hygromycin, Puromycin, G418, and others
   

For your convenience, we include complimentary samples of key assay reagents, such as the appropriate selection antibiotics and reporter detection reagents (e.g. QUANTI-Blue™, QUANTI-Luc™, or QUANTI-Star™) so you can begin your experiments immediately.

To assist in scalability, we offer cell banking services, providing extra vials of your custom clone(s).

Can’t find what you’re looking for? Contact us. We have customizable options for you.

Service step by step

Our streamlined process ensures a smooth and transparent experience from design to delivery. It is separated into two phases:
 

Service pathway step by step

Phase I - Molecular Design & Validation (8-10 weeks)

1. Contact - Reach out to tech@invivogen.com to discuss your project needs and cell line specifications. Together, we will define the project timeline and formalize the service agreement. Once approved, we proceed with the cell line development.

2. Molecular Construction - Our experts design and construct the plasmid and, if required, produce lentiviral vectors.

3. Pool Development - We perform stable transfection or transduction into your selected host cell line, validate gene expression, and assess specific activity. A progress report is prepared.

Phase II - Development & Delivery (12-15 weeks)

4. Monoclonal Cell Line Generation - We conduct cloning and sequential screening of multiple clones. The best-performing clones are validated, expanded, and cryopreserved.

5. Quality Control - The selected clone(s) undergo thawing and maintenance for up to 10 passages with expression and activity validation to ensure long-term stability.

6. Product Delivery - A final report is prepared, and the chosen cell line(s) are shipped to you. For your convenience, we also provide complimentary samples of essential assay reagents, such as selection antibiotics and reporter detection reagents.

Illustrations on this webpage are created with BioRender.com.

FAQ

Q: How much does a customized cell line from InvivoGen cost?

The cost of a customized cell line depends on multiple factors, including the complexity of genetic modifications, choice of the host cell line, licensing requirements, and additional validation steps. Just contact us, share your requirements, and we’ll provide a personalized quote.

Q: How long does InvivoGen's Custom Cell Line Service take?

The timeline varies depending on the complexity of the project and requests; it also depends on the cell line, growth characteristics, and culturing requirements. Typically, it takes our experts between 20 to 25 weeks, including cloning, selection, validation steps, reporting, and delivery.

Q: Is expedited service available if needed?

Yes, we offer expedited development options to shorten the timeline where possible. Contact us to discuss your specific requirements.

Q: Can I choose multiple clones and/or order multiple vials of the chosen clone(s)?

Absolutely. You can choose multiple clones. For your convenience and scalability, we offer cell banking services, allowing for the production and delivery of multiple vials of your chosen clone(s) upon request.

Q: Do you provide quality control testing?

Absolutely. We perform thorough QC testing, including sterility, mycoplasma detection, stability (up to 10 passages), expression validation, and functional assays to ensure high-quality results. 

Q: How stable are the custom cell lines over multiple passages?

We perform stability tests to guarantee stability for up to 10 passages. 

Q: What host cell lines do you recommend for my project?

Our tech support team can advise on the best host cell line based on your research application, whether you need an immune cell model, adherent cells, or suspension culture. We work with a wide range of mammalian cell lines, including HEK293, THP-1, Jurkat, A549, Raji, RAW-264, and B16 cell lines.

Q: Do I need additional reagents to start my experiments?

No. To streamline your workflow we include complimentary samples of essential assay reagents, such as suitable selection antibiotics and reporter detection reagents with your final product delivery.