EndoFit™ OVA protein

OVA protein for in vivo use

OVA (ovalbumin or albumin) is the most abundant protein in chicken egg whites. This glycoprotein is sufficiently large and complex to be mildly immunogenic.
 

Key features

Commercially available OVA is often contaminated with endotoxins which alter the results obtained in vivo [1]. InvivoGen ensures a high-quality OVA EndoFit™, for you to obtain accurate and reliable experimental results:

• Endotoxin levels <1 EU/mg
• Guaranteed sterile
• For in vivo use

Applications

• Model antigen for immunization experiments [2-4]
• Carrier protein for conjugation to haptens [5] and other antigens to increase their immunogenicity

InvivoGen also offers a large choice of vaccine adjuvants including alum & emulsions, PRR agonists, and conjugatable PRR ligands.

 For more information, read our review on Vaccine Adjuvants

References:

1. Watanabe J. et al., 2003. Endotoxin contamination of ovalbumin suppresses murine immunologic responses and development of airway hyperreactivity. J Biol Chem. 278(43):42361-8.
2. Lipford G.B. et al., 1993. Primary in vivo responses to ovalbumin. Probing the predictive value of the Kb binding motif. J Immunol. 150(4):1212-1222.
3. Newman M.J. et al., 1992. Saponin adjuvant induction of ovalbumin-specific CD8+ cytotoxic T lymphocyte responses. J Immunol. 148(8):2357-2362.
4. Vaz EM. et al., 1971. Persistent formation of reagins in mice injected with low doses of ovalbumin. Imunology, 21(1), 11-15.
5. Slütter B. et al., 2010. Conjugation of ovalbumin to N-trimethyl chitosan improves immunogenicity of the antigen. Journal of Controlled Release 143(2):207-14.

Specifications

Applications: immunization

CAS number: 9006-59-1

Molecular weight: ~ 45 kDa

Endotoxin level: < 1 EU/mg

Purity: minimum 98% protein (SDS-PAGE)

Solubility: 10 mg/ml in water

Contents

EndoFit™ OVA is provided lyophilized.

This product is available in two sizes:

• vac-pova

- 10 mg sterile EndoFit™ OVA
- 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

• vac-pova-100

- 100 mg (4 x 25 mg) sterile EndoFit™ OVA
- 2 x 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

EndoFit™ OVA contains <1 EU/mg endotoxins and no salts.

EndoFit™ OVA is shipped at room temperature.

Upon receipt, the product should be stored at 4°C.