ODN 2088

TLR9 Antagonist - Inhibitory ODN, preference towards mouse

Toll-like receptor 9 (TLR9) recognizes specific unmethylated CpG oligonucleotide (ODN) sequences that distinguish microbial DNA from mammalian DNA.

Studies suggest the existence of DNA sequences that can neutralize the stimulatory effect of CpG ODNs [1].

These sequences are characterized by three consecutive Gs downstream of a C or A, addition of a fourth G (G-tetrads) increases the inhibitory capability.

The most potent inhibitory sequences are (TTAGGG)4 found in mammalian telomeres [2] and ODN 2088 which derives from a stimulatory ODN by replacement of 3 bases [3].

Inhibitory ODNs act by disrupting the colocalization of CpG ODNs with TLR9 in endosomal vesicles without affecting cellular binding and uptake.

References:

1. Krieg AM. et al., 1998. Sequence motifs in adenoviral DNA block immune activation by stimulatory CpG motifs. Proc Natl Acad Sci USA. 95(21): 12631-6.
2. Gursel I. et al., 2003. Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation. J Immunol. 171(3):1393-400.
3. Stunz LL. et al., 2002. Inhibitory oligodinucleotides specifically block effects of stimulatory CpG oligonucleotides in B cells. Eur J Immunol. 32(5): 1212-22.

Specifications

Specificity: murine TLR9 antagonist

Working concentration: 100 nM - 10 µM

Solubility:  2.5 mg/ml in water

ODN 2088 sequence
5’- tcc tgg cgg gga agt -3’ (15 mer)
Note: Bases are phosphorothioate (nuclease resistant).

Quality control:

• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

ODN 2088 is provided lyophilized and is available in three quantities:

• 200 μg (41 nmol): tlrl-2088 (formerly tlrl-minhodn)
• 1 mg (205 nmol): tlrl-2088-5 (formerly tlrl-minhodn-5)
• 5 mg (1.025 mmol): tlrl-2088-5 (formerly tlrl-minhodn-5)
• 1.5 ml endotoxin-free water

ODN 2088 is shipped at room temperature.

Upon receipt, store at -20 °C.