Peptide M / Agarose

Agarose for IgA1 and IgA2 purification

Peptide M is a 50 aa synthetic peptide derived from a streptococcal M protein containing an additional C-terminal cysteine residue.

Peptide M binds monomeric and dimeric human IgA of both subclasses (IgA1 and IgA2) with high specificity and affinity.

It also binds bovine IgA but not murine IgA. Peptide M binding occurs at a site in IgA-Fc conserved in human IgA1 and IgA2 and bovine IgA but not in mouse IgA.

Peptide M can be used for single-step affinity purification of IgA and for specific detection of antigen-bound IgA.

Reference:

1. Sandin C. et al., 2002. Isolation and detection of human IgA using a streptococcal IgA binding peptide. J Immunol. 169(3):1357-64.

Specifications

Binding capacity: 4 - 6 mg human IgA per ml of gel

Contents

Peptide M / Agarose is available in two quantities:

• gel-pdm-2: 2 ml Peptide M / Agarose provided as a 50% v/v gel slurry in PBS 20% v/v ethanol (total volume 4 ml)
• gel-pdm-5: 5 ml Peptide M / Agarose provided as a 50% v/v gel slurry in PBS 20% v/v ethanol (total volume 10 ml)

Peptide M / Agarose is shipped at room temperature.

Store at 4 ̊C.

DO NOT FREEZE.