NOD2

Expression-ready ORF Clones

Alias : NLRC2, CARD15, IBD1, BLAU

Nucleotide-binding oligomerization domain containing 2.
Caspase recruitment domain-containing protein 15.

NOD2/CARD15 is an intracellular pattern-recognition protein involved in bacterial detection. Its expression is restricted to monocytes [1]. NOD2 is a general sensor of bacterial peptidoglycan (PGN). It recognizes the minimal bioactive fragment of PGN, called muramyl dipeptide, also known as MDP, a motif found in all PGNs [2].

This recognition event triggers a pro-inflammatory signaling cascade regulated by NF-kB. Mutations in NOD2 have been recently related to increased susceptibility to Crohn’s disease (CD). NOD2 deficiency increases TLR2-mediated activation of NF-κB resulting in a pro-inflammatory cytokine bias which could contribute to the overwhelming inflammation seen in CD [3,4].

References:

1. Ogura Y. et al., 2001. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB. J Biol Chem. 276(7):4812-8.
2. Girardin SE. et al., 2003. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem. 278(11):8869-72.
3. Watanabe T. et al., 2004. NOD2 is a negative regulator of Toll-like receptor 2-mediated T helper type 1 responses. Nat Immunol. 5(8):800-8.
4. Netea MG. et al., 2004. NOD2 mediates anti-inflammatory signals induced by TLR2 ligands: implications for Crohn’s disease. Eur J Immunol. 34(7):2052-9.

Specifications

Human NOD2 isoform 1 (pUNO1-hNOD2a)

ORF size : 3123 bp
Subclone : AgeI - NheI

Mouse NOD2 isoform 1 (pUNO1-mNOD2a)

ORF size : 3063 bp
Subclone : BspHI - NheI

pUNO1 Contents

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

Product is shipped at room temperature.

Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.