TICAM2-DN

Expression-ready ORF Clones

Alias: TRAM, TIRP

Toll-like receptor adaptor molecule 2
TRIF-related adapter molecule

Dominant negative TICAM2

TRIF-related adaptor molecule (TRAM), also known as TIR-containing adaptor molecule (TICAM)-2, is an adaptor molecule involved in the MyD88-independent TLR4 signaling pathway [1]. In response to LPS stimulation, TICAM2 physically bridges TLR4 and TRIF transmitting LPS-induced signaling to TRIF which in turn activates IRF-3 and the production of interferon beta [2]. TICAM2 function is restricted to the TLR4 pathway, as TICAM2-deficient mice are defective in cytokine production in response to TLR4 ligand but not to other TLR ligands [3].

The dominant negative TRAM/TICAM2 (C117H) was generated by mutating the cysteine at position 117 into a histidine. This TICAM2-DN was shown to retain its ability to bind to TLR4 but to lose its homo (TICAM2-TICAM2) and hetero (TICAM1-TICAM2) dimerizing properties [2].

1. Fitzgerald KA. et al., 2003. LPS-TLR4 Signaling to IRF-3/7 and NF-{kappa}B Involves the Toll Adapters TRAM and TRIF. J Exp Med. 198(7):1043-1055
2. Oshiumi H. et al., 2003. TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J Biol Chem. 278(50):49751-62
3. Yamamoto M. et al., 2003. TRAM is specifically involved in the Toll-like receptor 4-mediated MyD88-independent signaling pathway. Nat Immunol. 4(11):1144-50

Specifications

Human TICAM2-DN (pUNO1-hTICAM2-DN)

ORF size : 708 bp
Subclone : NcoI - NheI

pUNO1 Contents

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

Product is shipped at room temperature.

Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.