Lucia® luciferase Gene
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
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pSELECT-zeo-Lucia Lucia® luciferase Reporter Gene in expression plasmid |
Show product |
20 µg |
psetz-lucia
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Lucia® luciferase Reporter Gene in expression plasmid
InvivoGen provides the Lucia® secreted luciferase reporter gene in the pSELECT-zeo plasmid.
It can be used in vivo and in vitro to transfect mammalian cells stably or transiently.
Lucia® luciferase gene expression is driven by the EF-1α/HTLV composite promoter that combines the elongation factor 1 alpha core promoter and the 5’untranslated region of the Human T-cell Leukemia Virus.
The pSELECT-zeo-Lucia plasmid contains the Zeocin® resistance marker for selection in both mammalian cells and bacteria.
Note: Lucia® is a trademark of InvivoGen registered with the United States Patent and Trademark Office.
Back to the topSpecifications
Selectable with Zeocin® in both mammalian cells and bacteria.
Provided as lyophilized DNA.
These products are covered by a Limited Use License (See Terms and Conditions).
Back to the topContents
Lucia® luciferase reporter gene is provided as 20 µg of lyophilized DNA.
It is supplied with:
- 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia® luciferase detection reagent (sufficient to prepare 25 ml)
- 1 ml of Zeocin® (100 mg/ml)
Product is shipped at room temperature.
Description
High signal sensitivity.
The human embryonic kidney HEK293, the murine melanoma B16 and the murine macrophage RAW 264.7 cell
lines were transiently transfected with 0.1, 0.3 or 1 µg/ml of pSELECT-Lucia-zeo plasmid using the transfection reagent LyoVec™.
Forty-eight hours after transfection, cell supernatants were assessed for luciferase activity using QUANTI-Luc™ 4 Lucia/Gaussia.
