LyoVec™
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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LyoVec™ Cationic lipid for transfection of mammalian cells (2 ml vials) |
Show product |
10 ml, 200 reactions 20 ml, 400 reactions |
lyec-1
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LyoVec™ (with control) Cationic lipid for transfection of mammalian cells (2 ml vials) + 1 vial of control LyoVec™-GFP/SEAP complex (1 ml vial) |
Show product |
8 ml, 160 reactions 18 ml, 360 reactions |
lyec-12
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Lyophilized Lipid-based Transfection Reagent
LyoVec™ is a proprietary cationic lipid-based transfection reagent belonging to the family of phosphonolipids. This family was originally described by Floch et al., as efficient transfection reagents for both in vitro and in vivo use [1-2].
BULK QUANTITY:The major constituent of LyoVec™ is the phosphonolipid DTCPTA, which is coupled to DiPPE, a neutral lipid that helps destabilize membrane bilayers and increases the in vitro transfection efficiency of LyoVec™.
Applications of LyoVec™:
- Cationic lipid-based transfection reagent
- Nucleic acid complexing agent
The positive charge of the phosphonolipid enables LyoVec™ to bind to plasmid DNA, and the structure promotes fusion with cellular membranes for efficient DNA delivery.
Additionally, LyoVec™ can be used as a nucleic acid complexing agent to facilitate the cellular entry of RNA or DNA-based oligonucleotides, such as RIG-I ligands (e.g. poly(dA:dT) [3] and poly(I:C) [4]) and endosomal TLR7/8 ligands (e.g. ssPolyU [5]). This complexation step is crucial for inducing an effective response to the nucleic acids by pathogen recognition receptors.
References
1. Floch et al., 1997. Cationic phosphonolipids as non-viral vectors for DNA transfection in hematopoietic cell lines and CD34+ cells. Blood Cells Molec.& Diseases 23:69-87.
2. Guillaume-Gable et al., 1998. Cationic phosphonolipids as nonviral gene transfer agents in the lung of mice. Hum Gene Ther 9:2309-2319.
3. Xie M. et al., 2016. PKM2-dependent glycolysis promotes NLRP3 and AIM2 inflammasome activation. Nat Commun. 7:13280.
4. Järver P. et al., 2018. Single-Stranded Nucleic Acids Regulate TLR3/4/7 Activation through Interference with Clathrin-Mediated Endocytosis. Sci Rep. 8:15841.
5. Katashiba et al., 2011. Interferon-α and interleukin-12 are induced, respectively, by double-stranded DNA and single-stranded RNA in human myeloid dendritic cells. Immunology. 132(2):165-73.
Specifications
Hands-on time: No more than 15 min and no preparation required the day prior transfection
Transfection: Transient and stable transfections
Conditions: Works in the presence of serum, with no need to wash cells after transfection
Shelf life: Long shelf-life of plasmid DNA (pDNA)/ LyoVec™ complexes
Quality Control:
- Transfection efficiency of LyoVec™ has been confirmed in HEK293‑derived cells.
- Cytotoxicity has been assessed in HEK293‑derived cells.
Contents
LyoVec™ is provided as a lyophilized powder, sterile, and packaged in sealed glass vials.
This product is available in different formats:
- lyec-1 : 5 small vials (sufficient to perform 200 reactions in 12-well plates)
- lyec-2 : 10 small vials (sufficient to perform 400 reactions in 12-well plates)
- lyec-12 : 4 small vials (sufficient to perform 160 reactions in 12-well plates) and 1 vial of control LyoVec™-GFP/SEAP complex.
- lyec-22 : 9 small vials (sufficient to perform 360 reactions in 12-well plates) and 1 vial of control LyoVec™-GFP/SEAP complex.
LyoVec™ is shipped at room temperature.
Upon receipt, it should be stored at 4°C.