pDRIVE-Tyr / Tyr
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDRIVE-Tyr-mTyr pDRIVE bearing mouse Tyr / Tyr promoter - LacZ |
Show product |
20 µg |
pdrive-tyrmtyr
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pDRIVE5SEAP-Tyr-mTyr pDRIVE bearing mouse Tyr / Tyr promoter - SEAP |
Show product |
20 µg |
pdrive5s-tyrmtyr
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pDRIVE5Lucia-Tyr-mTyr pDRIVE bearing mouse Tyr / Tyr promoter - Lucia |
Show product |
20 µg |
pdrive5lc-tyrmtyr
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Tyrosinase enhancer and promoter in pDRIVE expression plasmid
Tyrosinase, the rate-limiting enzyme in melanin synthesis, is expressed specifically in pigment-producing cells.
The expression of tyrosinase is regulated by cyclic AMP (cAMP). The M-box 70-bp upstream from the TATA-box and the E-box located downstream of the TATA-box, near the initiator site, are involved in the regulation of the tyrosinase promoter activity by cAMP [1].
Melanoma-specific expression of a transgene can be achieved by using the tyrosinase promoter [2], this targeted expression being further amplified by the addition of the melanocyte-specific enhancer.
References:
1. Bertolotto C. et al. 1996. Regulation of tyrosinase gene expression by cAMP in B16 melanoma cells involves two CATGTG motifs surrounding the TATA box: implication of the microphthalmia gene product. J Cell Biol. 134(3):747-55.
2. Vile R. et al. 1994. A comparison of the properties of different retroviral vectors containing the murine tyrosinase promoter to achieve transcriptionally targeted expression of the HSVtk or IL-2 genes. Gene Ther. 1(5):307-16.
Specifications
pDRIVE bearing mouse Tyr / Tyr promoter - LacZ, SEAP or Lucia
Gene Promoter: Tyr : Tyrosinase
Specificity: Melanocytes & melanoma
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
pDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
