Pam3CSK4 VacciGrade™

Synthetic triacylated lipopeptide - TLR2-based adjuvant

Pam3CSK4 VacciGrade™ is a synthetic lipopeptide and a TLR2/1 ligand.  Its recognition is mediated by TLR2 which cooperates with TLR1 through their cytoplasmic domain to induce the signaling cascade leading to NF-κB activation.

Pam3CSK4 mimics the acylated amino terminus of bacterial lipopeptides, a family of pro-inflammatory cell wall components found in both Gram-positive and Gram-negative bacteria. Their acylated amino terminus confers them with TLR2 stimulatory activity.

Pam3CSK4 is a potent adjuvant for various vaccines, including flu and leishmaniasis vaccines. Pam3CSK4 increases antibody responses to flu antigens and upregulates proinflammatory and Th1 cytokine genes [1]. Pam3CSK4 also improves the efficacy of a DNA-based vaccine against Leishmania [2]. 

Pam3CSK4 VacciGrade™ is a high-quality pre-clinical grade.
Pam3CSK4VacciGrade™​ is for research use only, and not for human or veterinary use.

References:

1. Caproni E. et al., 2012. MF59 and Pam3CSK4 Boost Adaptive Responses to Influenza Subunit Vaccine through an IFN Type IIndependent Mechanism of Action. J Immunol. 188(7):3088-98
2. Jayakumar A. et al., 2011. TLR1/2 activation during heterologous prime-boost vaccination (DNA-MVA) enhances CD8+ T Cell responses providing protection against Leishmania (Viannia). PLoS NeglTrop Dis. 5(6):e1204.

Specifications

Description: TLR2 agonist VacciGrade™

Polarization of adaptive immune response: Th1 response

CAS number: 112208-01-2

Molecular formula: C₈₁H₁₅₆N₁₀O₁₃S • 3TFA

Molecular weight: 1852.33 g/mol

Solubility: 1 mg/ml in physiological water

Working concentration: 2 -20 µg/mouse

Quality control: 

• Sterility guaranteed
• The absence of other bacterial contamination (lipoproteins & endotoxins) has been confirmed using HEK-Blue™ TLR4 cells
• Endotoxin level < 50 EU/mg (measurement by kinetic chromogenic LAL assay)

VacciGrade™

VacciGrade™ is a high-quality pre-clinical grade. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room*. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and/or a TLR2 and TLR4 reporter assay.
*Except for LPS VacciGrade™, which is prepared in a laminar flow hood dedicated to LPS.

Contents

• 1 mg of lyophilized Pam3CSK4 VacciGrade™
• 1.5 ml endotoxin-free water
• 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Pam3CSK4 VacciGrade™ is shipped at room temperature

Pam3CSK4 VacciGrade™ should be stored at 4°C.

Lyophilized product is stable 1 year when properly stored.