CFA

Vaccine Adjuvant: Complete Freund’s Adjuvant

Complete Freund's Adjuvant (CFA) consists of heat-killed Mycobacterium tuberculosis in non-metabolizable oils (paraffin oil and mannide monooleate). CFA contains trehalose 6,6’ dimycolate (TDM), which stimulates Mincle. Additionally, CFA has ligands for TLR2, TLR4, and TLR9.

CFA can be used to produce water-in-oil emulsions of antigens.

Injection of antigen in CFA induces a Th1-dominated response when compared to injection in Incomplete Freund's Adjuvant (IFA), which lacks mycobacterial components and induces a Th2-dominated response.

An essential outcome of the CFA-mediated response is an intense inflammatory reaction at the site of antigen deposition. For most applications, CFA is usually only necessary for the initial immunization, while IFA is the adjuvant of choice for subsequent immunizations.

CFA is VacciGrade™, a high-quality pre-clinical grade.

CFA is for research use only, and not for human or veterinary use.

Specifications

Specificity: Th1 response

VacciGrade™ (preclinical grade)

Quality control

• Sterility guaranteed
• Endotoxin level < 1 EU/ml (measurement by kinetic chromogenic LAL assay)
• Adjuvanticity of CFA was evaluated by assessing the levels of total mouse IgGs (mIgGs) and the mIgG1 and mIgG2 isotypes after an initial subcutaneous injection of EndoFit™ Ovalbumin/CFA (1:1, v/v) in mice. To minimize side effects, EndoFit™ Ovalbumin/IFA (1:1, v/v) was used for a subcutaneous boost injection. Results were compared to mice which received the antigen alone.

Contents

CFA is available in two quantities:

• 10 ml: cat. code # vac-cfa-10
• 6 x 10 ml: cat. code # vac-cfa-60

Each 10 ml CFA vial contains 10 mg heat killed dried Mycobacterium tuberculosis (strain H37Ra), 1.5 ml Mannide Monooleate and 8.5 ml Paraffin oil.

CFA is shipped at room temperature.

CFA should be stored at 4˚C.

CFA is stable for 2 years.

VacciGrade™

VacciGrade™ is a high-quality pre-clinical grade. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room*. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and/or a TLR2 and TLR4 reporter assay.
*Except for LPS VacciGrade™, which is prepared in a laminar flow hood dedicated to LPS.