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HT29-Lucia™ AhR Cells

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HT29-Lucia™ AhR Cells

Human HT29 colon adenocarcinoma - AhR reporter cells

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3-7 x 10e6 cells

ht2l-ahr
+-
$1,320

AhR signaling pathway
AhR signaling pathway

Human HT29 colon adenocarcinoma - AhR reporter cells

HT29-Lucia™ AhR cells are engineered from the human HT-29 colon adenocarcinoma cell line for the study of the aryl hydrocarbon receptor (AhR) genomic signaling induction (see details), by monitoring the activity of the Lucia luciferase reporter protein.

AhR is a ligand-dependent transcriptional factor widely expressed in barrier tissues. Notably, AhR plays an important role in gut microbiota and the host's immune homeostasis [1].

HT-29 cells are characterized by:

  • Endogenous AhR expression [2]
  • Ability to differentiate into intestinal enterocyte-like cells and mucus-producing cells [3]

Therefore, HT29-Lucia™ AhR cells are highly relevant for studying intestinal microbiota-related ligands for AhR.

HT29-Lucia™ AhR cells express the secreted Lucia luciferase reporter gene under the control of a minimal promoter coupled with the human Cyp1a1 gene's entire regulatory sequence, which contains six dioxin-responsive elements (DREs).
As a result, these cells allow studying the AhR genomic signaling pathway, by monitoring the activity of Lucia luciferase in the cell culture supernatant when using the QUANTI-Luc™ 4 Lucia/Gaussia detection reagent.

HT29-Lucia™ AhR cells are resistant to Zeocin®.

 

Read our review Read our review on AhR's key role in intestinal microbiota and immunity.

 

References

1. Lamas B et al.< 2018. Aryl hydrocarbon receptor and intestinal immunity. Mucosal Immunol. 11:1024-38.
2. Park JH. et al., 2018. Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. Oncol Rep. 39(4):1930-8. 
3 artínez-Maqueda D. et al. 2015. HT29 Cell Line. Bioactives on Health: in vitro and ex vivo models. Springer. Chapt 1.

Figures

Evaluation of AhR-induced responses in HT29-Lucia™ AhR cells.
Evaluation of AhR-induced responses in HT29-Lucia™ AhR cells.

Induction of AhR activity by tryptophan byproducts in HT29-Lucia™ AhR cells. The cells were incubated with 100 μM ITE, 18 μM FICZ, or 150 μM L-Kynurenine. After overnight incubation, the AhR activation was assessed by determining Lucia luciferase activity in the supernatant using QUANTI-Luc™. Data are expressed as fold responses as compared to non-induced cells.

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Specifications

Antibiotic resistance: Zeocin®

Quality Control:

  • Reporter activity has been verified by functional assays.
  • The stability of this cell line for 20 passages following thawing has been verified.
  • HT29-Lucia™ AhR cells are guaranteed mycoplasma-free.
     

For internal research use for non-profit recipients only.

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Contents

  • 3-7 x 106 of HT29-Lucia™ AhR cells in a cryovial or shipping flask
  • 1 ml Zeocin® (100 mg/ml)
  • 1 ml Normocin™ (50 mg/ml)
  • 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)

Shipped on dry ice Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

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Details

The aryl hydrocarborn receptor (AhR) is a ligand-dependent transcriptional factor widely expressed in barrier tissues [1]. AhR plays a key role in gut-microbiota and host’s immune homeostasis, not only in the intestine but also at distant sites [1].
Besides xenobiotics, including the prototypic AhR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a variety of dietary-derived AhR ligands have been identified, many of which are byproducts of tryptophan (Trp) metabolism [2].

Inactive AhR resides in the cytoplasm within a Hsp90:XAP2:p23:Src protein complex. The AhR canonical genomic signaling pathway occurs as follows: upon ligand binding, the complex undergoes conformational changes and translocates into the nucleus. AhR heterodimerizes with AhR nuclear translocator (ARNT) before binding to dioxin response elements (DREs) in the upstream regulatory regions of AhR target genes, such as the cytochrome P450-dependent monooxygenase Cyp1a1, the AhR repressor (AhRR), and the IL-22 interleukin.


Of note, non-canonical AhR signaling pathways have also been reported, either at the genomic level through association with other transcription factors (e.g. NF-κB), or at the non-genomic level (e.g. through the release of the Src kinase) [2,3].

 

 

References

1. Lamas B. et al. 2018. Aryl hydrocarbon receptor and intestinal immunity. Mucosal Immunol. 11:1024-38.
2. Gao J. et al. 2018. Impact of the gut microbiota on intestinal immunity mediated by tryptophan metabolism. Front. Cell. Infect. Microbiol. 8:13.
3. Park JH. et al., 2018. Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. Oncol Rep. 39(4):1930-8. 

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Notification:  This product is for internal research use for non-profit recipients only.

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