Recombinant human TSLP protein - Bioactive cytokine

Human TSLP protein - Mammalian cell-expressed, His-tag, carrier-free

Recombinant human TSLP is a high-quality and biologically active cytokine, validated using proprietary TSLP reporter cells. This IL-7-like cytokine and member of the IL-2/γc superfamily is produced in CHO cells to ensure protein glycosylation and bona fide 3D structure.

Recombinant human TSLP can be used together with HEK-Blue™ TSLP cells for the screening of inhibitory molecules, such as Tezepelumab, a monoclonal antibody targeting TSLP (see figures).

TSLP signaling and biological functions

Key features

• Each lot is validated using HEK-Blue™ TSLP cells
• Endotoxin ≤ 0.1 EU/µg
• 0.2 µm sterile-filtered

Applications

• Standard for TSLP detection and quantification assays
• Screening and release assays for antibodies blocking TSLP signaling
• Screening and release assays for engineered TSLP

Thymic stromal lymphopoietin (TSLP) is a critical mediator of type 2 (T2) immune responses, acting at the early stages of inflammation. Abnormal TSLP expression is linked to immune-mediated inflammation diseases, notably asthma and allergic airway inflammation.

 More details

All InvivoGen products are for internal research use only, and not for human or veterinary use.

Figures

SDS-PAGE analysis of recombinant human (h)TSLP. 1.5 μg of hTSLP was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. A band was detected at ~20 kDa.

Dose-response of HEK-Blue™ TSLP cells to recombinant human TSLP. HEK-Blue™ TSLP cells were stimulated with increasing concentrations of recombinant human TSLP. After overnight incubation, the STAT5-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Dose-dependent inhibition of HEK-Blue™ TSLP cell response using Tezepelumab biosimilar. Increasing concentrations of Anti-hTSLP-hIgG2 (0.3 ng/ml - 30 µg/ml) were incubated with recombinant human TSLP (1 ng/ml) for 1 h before the addition of HEK-Blue™ TSLP cells. After overnight incubation, SEAP activity in the cell culture supernatant was assessed using QUANTI-Blue™ Solution. Data are shown as the percentage of activity (mean ± SEM).

Specifications

Source: Chinese hamster ovary (CHO) cells

Species: Human

Alternative name: n/a

Carrier: Carrier-free

Tag: Hexahistidine (His6)

UniProt ID: Q969D9

Protein sequence: 131 a.a. (Y29-Q159 with R127A and R130S mutations)

Molecular weight: ~ 19.9 kDa (SDS-PAGE)

Solubility: 100 μg/ml in water

Formulation: Phosphate buffer saline (pH 7.4), 5% saccharose

Sterility:  0.2 µm filtration

Form:  Lyophilized

Reconstitution buffer:  Endotoxin-free water (provided)

Purity:  ≥93% (SDS-PAGE)

Endotoxin level:  ≤ 0.1 EU/μg (measurement by kinetic chromogenic LAL assay)

Tested applications:  Cellular assays

Quality control: Each lot is functionally tested and validated.

Contents

Recombinant human TSLP is provided lyophilized and is available in two quantities:

• rcyc-htslp-hs: 10 µg
• rcyc-htslp-hs-5: 5 x 10 µg
• 1.5 ml endotoxin-free water for rcyc-htslp-hs and rcyc-htslp-hs-5

 Recombinant hTSLP is shipped at room temperature.

 Upon receipt, the product should be stored at -20°C.

 Avoid repeated freeze-thaw cycles.

Details

Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7)-like cytokine with multiple functions, including cell proliferation and survival. TSLP binds a heterodimeric transmembrane receptor comprising the shared IL-7 receptor alpha-chain (IL-7Rα, CD127) and the TLSP receptor chain (TSLPR, CRLF2), a γc-like subunit. It signals through tyrosine kinases of the Janus family (JAK1 and JAK2) and signal transducer and transcription activators (STATs), notably STAT5 [1]. TSLP is best known as a critical mediator of type 2 immune responses and a promoter of Th2 cell-mediated diseases (e.g. asthma) [1,2].

References:

1. Ebina-Shibuya R. & Leonard WJ., 2023. Role of thymic stromal lymphopoietin in allergy and beyond. Nat Rev Immunol, 23(1):24-37.
2. Schmitt P. et al., 2024. TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation. J Exp Med. 221(6) e20231236.