Recombinant human IL-1β protein

Recombinant human interleukin 1 beta protein - produced in CHO cells or E. coli

InvivoGen offers two recombinant versions of the mature human (h) interleukin 1 beta (IL-1β), expressed in

– Chinese hamster ovary (CHO) cells  -  carrier-free, or 

– Escherichia coli (E. coli) - with HSA carrier.

Interleukin 1 beta (IL-1β) is a secreted pro-inflammatory cytokine that generates systemic and local responses to infection and injury. Human IL-1β is produced as a precursor (269 amino acids) cleaved intracellularly by the cysteine protease Caspase-1 to generate the active cytokine. IL-1β is a non-glycosylated polypeptide chain containing 153 amino acids with a molecular mass of 17 kDa. 

 More details

InvivoGen's IL-1β cytokines can be used to induce its cognate signaling pathway in HEK-Blue™ IL-1β cells (see figure). The size and purity were assessed using SDS PAGE. The predicted size of human IL-1β is ~17 kDa. The higher band in the SDS-page of the CHO-derived IL-1β at ~19 kDa corresponds to the glycosylated protein (see figures). 

Key features

• Produced in CHO cells or  E. coli
• High-quality: purity ≥ 95% and endotoxin level < 1EU/µg
• Functionally tested

Applications

• Cellular assays
• Cell culture
• ELISA

Figures

SDS PAGE of the recombinant human (h)IL-1β protein. 3 μg of CHO-derived hIL-1β was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. Its predicted size is ~17 kDa (lower band). The higher band at ~19 kDa corresponds to the glycosylated protein. 

Dose-response in HEK-Blue™ IL-1β cells to recombinant IL-1β cytokines of different sources. Cells were stimulated with increasing concentrations of recombinant human IL-1β, derived from E. coli (blue) or CHO cells (red). After overnight incubation, the NF-κB-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source:

• rcyc-hil1b: produced in Chinese hamster ovary (CHO) cells
• rcyec-hil1b: produced in E. coli

Alternative name: IL1F2, Interleukin 1 beta

UniProt ID: P01584

Protein sequence: Ala117-Ser269

Molecular mass: ~ 17 kDa

Solubility: 100 μg/ml in water

Formulation: 

• Human IL-1β produced in CHO cells: Lyophilized from a 0.2 μm filtered solution in 20 mM Sodium Phosphate, 150 mM NaCl, and 5% saccharose buffer solution (pH 7)
• Human IL-1β produced in E. coli: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7) with human serum albumin (HSA) as a carrier protein 

Quality control:

• Purity: ≥95% (SDS-PAGE)
• Endotoxin Level: ≤ 0.1 EU/μg (measurement by kinetic chromogenic LAL assay)
• The biological activity has been confirmed using HEK-Blue™ IL-1β cells.

Contents

Note: Each product is sold separately.

Recombinant human IL-1β (produced in CHO cells) - Carrier-free

• 10 μg of lyophilized recombinant human IL-1β (CHO)
• 1.5 ml endotoxin-free water.

Recombinant human IL-1β (produced in E. coli) - Carrier HSA

• 10 μg of lyophilized recombinant human IL-1β (E. coli)
• 1.5 ml endotoxin-free water.

Recombinant human IL-1β is shipped at room temperature.

Upon receipt, it should be stored at -20°C.

Details

Interleukin 1β (IL-1β) is a soluble pro-inflammatory cytokine that plays a critical role in the host response to infection and injury [1]. It is synthesized as a pro-IL-1β zymogen by activated macrophages and must be cleaved by caspase-1 to generate its mature form [2]. IL-1β binding to the IL-1R1 receptor triggers the formation of the IL-1R1/IL-1R3/MyD88 complex and induces signaling leading to the activation of the transcription factors NK-κB and AP-1 [3].

Due to its role in mediating acute and chronic inflammation, IL-1β has emerged as a therapeutic target for auto-inflammatory diseases [1,4]. 

1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27. 
2. Lopez-Castejon G. & Brough D., 2011. Understanding the mechanism of IL-1β secretion. Cytokine Growth Factor Rev. 22(4):189-95.
3. Weber A. et al., 2010. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.
4. Dinarello CA., 2011. Interleukin-1 in the pathogenesis and treatment of inflammatory diseases. Blood. 117:3720–3732.