Recombinant human CD40L protein - Bioactive cytokine

Human CD40L protein - Mammalian cell-expressed, tag-free, with HSA

Recombinant human CD40L is a high-quality and biologically active cytokine, validated using proprietary CD40L reporter cells. This member of the TNF superfamily is produced in E. coli and thoroughly purified to remove endotoxins.

Recombinant human CD40L can be used together with HEK-Blue™ CD40L cells for the screening of inhibitory molecules, such as Frexalimab, a monoclonal antibody targeting CD40L (see figures).

CD40L signaling and biological functions

Key features

• Each lot is validated using HEK-Blue™ CD40L cells
• Endotoxin < 0.1 EU/µg
• 0.2 µm sterile-filtered

Applications

• Standard for CD40L detection and quantification assays
• Screening and release assays for antibodies blocking CD40L signaling
• Screening and release assays for engineered CD40L

CD40 Ligand (CD40L) is a protein that is primarily expressed on activated T cells and is a member of the tumor necrosis factor superfamily. Of note, CD40L, together with its receptor CD40, plays a pivotal role in cellular and humoral immunity.

 More details

InvivoGen’s products are for research use only, and not for clinical or veterinary use.

Figures

SDS PAGE of the recombinant human (h)CD40L protein. 1.5 μg of hCD40L was loaded on a 12% Mini-PROTEAN® TGX StainFree™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. A band was detected at ~16 kDa.

Dose-response in HEK-Blue™ CD40 cells to recombinant hCD40L protein. Cells were stimulated with increasing concentrations of recombinant human (h)CD40L. After overnight incubation, the NF-κB-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source: E. coli

Species: Human

Alternative names: CD154, T-cell antigen Gp39, TNF-related activation protein (TRAP), Tumor necrosis factor ligand superfamily member 5

Carrier: HSA (human serum albumin)

Tag: Tag-free

Accession number: P29965

Molecular weight: ~ 16 kDa (SDS-PAGE)

Solubility: 100 μg/ml in water

Formulation: Phosphate buffer saline (pH 7.4), 5% saccharose, 2% HSA

Sterility: 0.2 µm filtration

Form: Lyophilized

Reconstitution buffer: Endotoxin-free water (provided)

Purity: ≥95% (SDS-PAGE)

Endotoxin level: ≤ 0.1 EU/μg (measurement by kinetic chromogenic LAL assay)

Tested applications: Cellular assays

Quality control: Each lot is functionally tested and validated.

Contents

• 10 μg of recombinant human CD40L provided as a white lyophilized powder
• 1.5 ml endotoxin-free water.

Recombinant human CD40L is shipped at room temperature.

Upon receipt, the product should be stored at -20 °C.

 Avoid repeated freeze-thaw cycles.