Recombinant human IL-6 protein

Recombinant human interleukin-6 protein (with HSA)

Recombinant human interleukin 6 (IL-6) is a single, nonglycosylated, polypeptide chain containing 185 amino acids. IL-6 has a molecular weight of 21kDa. IL-6 is produced in Escherichia coli.

IL-6 is used to induce its cognate signalling pathway in HEK-Blue™ IL-6.

Figures

SDS PAGE of the recombinant human (h)IL-6 protein. 1.5 μg of hIL-6 was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad) stained with Coomassie. Detection was performed as per the manufacturer’s instructions. A band was detected at ~21 kDa

Dose-response in HEK-Blue™ IL-6 cells to recombinant IL-6 cytokine. Cells were stimulated with increasing concentrations of recombinant human IL-6. After overnight incubation, the NF-κB-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source: E. coli

UniProt ID: P05231

Formulation: Lyophilized from a 0.2 µm filtered phosphate buffer solution (pH 7.4) containing human serum albumin (HSA).

Purity: >95%, by SDS-PAGE.

Molecular mass: ~ 21 kDa

Solubility: 100 μg/ml in water.

Endotoxin level: Less than 0.1 EU/µg as determined by the LAL method.

Bioactivity: Validated using HEK-Blue IL6 cells.

Specific activity: IL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 5 x 10⁷ IU/mg.

Contents

• 10 μg of IL-6 provided as a white lyophilized powder.
• 1.5 ml endotoxin-free water.

IL-6 is shipped at room temperature.

Upon receipt it should be stored at -20°C.