RAW-Lucia™ ISG-KO-TREX1 Cells
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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RAW-Lucia™ ISG-KO-TREX1 Cells TREX1 Knockout IRF-Lucia Luciferase Reporter Cell Line |
Show product |
3-7 x 10e6 cells |
rawl-kotrex
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TREX1 Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages
RAW-Lucia™ ISG-KO-TREX1 cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the TREX1 gene. TREX1 (also known as DNAse III) is a major cellular 3’5’ exonuclease that plays a crucial role in maintaining immune homeostasis.
RAW-Lucia™ ISG-KO-TREX1 and RAW-Lucia™ ISG cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
RAW-Lucia™ ISG-KO-TREX1 and RAW-Lucia™ ISG cells can be used to study the role of TREX1 by monitoring IRF-induced Lucia luciferase activity. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
The response of RAW‑Lucia™ ISG-KO-TREX1 cells to murine type I interferons (IFNs) is unaffected by the knockout of the TREX1 gene. The TREX1 KO appears to have little to no effect on the response to the ligands tested (e.g. VACV70/LyoVec and 2'3'-cGAMP).
RAW-Lucia™ ISG-KO-TREX1 cells are resistant to Zeocin®.
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Antibiotic resistance: Zeocin®
Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™ supplemented with Zeocin®
Quality Control:
TREX1 knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion.
The cells are guaranteed mycoplasma-free.
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- 1 vial containing 3-7 x 106 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
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