THP1-Blue™ ISG Cells

Interferon regulatory factor-inducible SEAP Reporter Monocytes

THP1-Blue™ ISG cells were specifically designed for monitoring the interferon signaling pathway in a physiologically relevant cell line.

THP1-Blue™ were derived from the human THP-1 monocyte cell line by stable integration of an interferon regulatory factor (IRF)-inducible SEAP reporter construct.

As a result, THP1-Blue™ ISG cells allow the monitoring of IRF activation by determining the activity of SEAP.

The levels of IRF-induced SEAP in the cell culture supernatant are readily assessed with QUANTI-Blue™, a SEAP detection reagent.

THP1-Blue™ ISG cells are highly responsive to PRR agonists that trigger the IFN signaling pathway, such as LPS and transfected double-stranded nucleic acid.

Figures

Cells were incubated with 10 ng/ml Pam3CSK4 (TLR1/2), 10 µg/ml poly(I:C) (TLR3), 100 ng/ml LPS-EB UP (TLR4), 1 µg/ml FLA-ST UP (TLR5), 10 µg/ml R848 (TLR7/8), 100 ng/ml poly(I:C)/LyoVec (RLR), 10 ng/ml poly(dA:dT)/LyoVec (RLR), 100 ng/ml 5’pppRNA/Lyovec (RLR), 10 ng/ml TNF-α or 10 IU/ml IFN-α. After 24h incubation, the levels of IRF-induced SEAP were assessed from the cell culture supernatant using QUANTI-Blue™.

Specifications

Antibiotic resistance: Zeocin®

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Quality Control:

• THP1-Blue™ ISG cells produce SEAP following stimulation with PRR agonists that trigger the IFN signaling pathway, such as LPS and transfected double-stranded nucleic acid.
• THP1-Blue™ ISG cells are guaranteed mycoplasma-free.

Contents

• 1 vial containing 3-7 x 10⁶ cells
• 1 ml Normocin™ (50 mg/ml)
• 1 ml of Zeocin® (100 mg/ml)
• 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)

Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

Description

THP1-Blue™ were derived from the human THP-1 monocyte cell line by stable integration of an interferon regulatory factor (IRF)-inducible SEAP reporter construct. 
THP1-Blue™ ISG cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. 
As a result, THP1-Blue™ ISG cells allow the monitoring of IRF activation by determining the activity of SEAP.

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